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surescan high-resolution dna microarray scanner  (Agilent technologies)


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    Structured Review

    Agilent technologies surescan high-resolution dna microarray scanner
    Flow chart of <t>microarray</t> data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.
    Surescan High Resolution Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+microarray+scanner/bio_rxiv__2024__06__24__600143-52-12-11
    Average 90 stars, based on 1 article reviews
    surescan high-resolution dna microarray scanner - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis"

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    Journal: bioRxiv

    doi: 10.1101/2024.06.24.600143

    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.
    Figure Legend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Techniques Used: Microarray, Software

    Related Articles

    Microarray:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Construct:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Mutagenesis:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Sequencing:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Binding Assay:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Hybridization:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Quantitative RT-PCR:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Expressing:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Derivative Assay:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Spectrophotometry:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Labeling:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Incubation:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Software:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.

    Extraction:

    Article Title: Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules
    Article Snippet: After hybridization, slides were washed for 5 minutes at room temperature with CGH Wash Buffer 1 (Agilent) and 1 minute at 37°C with CGH Wash buffer 2 (Agilent) and scanned immediately, using an Agilent High Resolution Microarray Scanner, at 2 μm resolution, 100% PMT.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis
    Article Snippet: Hybridized chips were scanned on an Agilent High Resolution Microarray Scanner.

    Article Title: Global transcriptional response of Caulobacter crescentus to iron availability
    Article Snippet: The arrays were scanned for the Cy3 and Cy5 fluorescent signals with an Agilent High Resolution Microarray Scanner.

    Article Title: Decellularized tissue engineered hyaline cartilage graft for articular cartilage repair.
    Article Snippet: Articular cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of cartilage tissue.. Currently, cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI).. However, these treatments are frequently reported to result in a mixture of the desired hyaline cartilage and mechanically inferior fibrocartilage.

    Article Title: Insulin Enhances Migration and Invasion in Prostate Cancer Cells by Up-Regulation of FOXC2.
    Article Snippet: Scanning was performed using Agilent High Resolution Microarray Scanner, and data was processed with the Agilent Feature Extraction 10.5.1 software (Agilent).

    Article Title: MicroRNA‑142‑3p inhibits trophoblast cell migration and invasion by disrupting the TGF‑β1/Smad3 signaling pathway.
    Article Snippet: After passing the RNA purity and quantity measurement using the NanoDrop ND‐1000 Spectrophotometry (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and Agilent's 2100 Bioanalyzer, samples were labeled with miRCURYTM Hy3TM/ Hy5TM Power labeling kit and hybridized to the miRCURY LNATM Array (v.18.0; Agilent Technologies, Inc., Santa Clara, CA, USA), incubated, washed and scanned in Agilent high resolution microarray scanner.



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    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software

    The 11 genes were detected in the microarray analysis and in the human dataset. A correlation between FCs of genes. B heatmap with hierarchical clustering of FCs of the 11 genes commonly detected in the murine neurospheres and AD patients’ tissues

    Journal: Cell & Bioscience

    Article Title: Bradykinin promotes immune responses in differentiated embryonic neurospheres carrying APP swe and PS1 dE9 mutations

    doi: 10.1186/s13578-024-01251-3

    Figure Lengend Snippet: The 11 genes were detected in the microarray analysis and in the human dataset. A correlation between FCs of genes. B heatmap with hierarchical clustering of FCs of the 11 genes commonly detected in the murine neurospheres and AD patients’ tissues

    Article Snippet: The arrays were then washed as described on Agilent SSPE wash protocol v. 2.1 and scanned with a High-Resolution Microarray Scanner (Agilent Technologies).

    Techniques: Microarray